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A novel method to detect hPG80 (human circulating progastrin) in the blood
Sep, 2021
Journals - Analytical Methods - 2021, 13, 4468 – 4477
Abstract
hPG80 (human circulating progastrin) is produced and released by cancer cells. We recently reported that hPG80 is detected in the blood of patients with cancers from different origins, suggesting its potential utility for cancer detection. To accurately measure hPG80 in the blood of patients, we developed the DxPG80 test, a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). This test quantifies hPG80 in EDTA plasma samples. The analytical performances of the DxPG80 test were evaluated using standard procedures and guidelines specific to ELISA technology. We showed high specificity for hPG80 with no cross-reactivity with human glycine-extended gastrin (hG17-Gly), human carboxy-amidated gastrin (hG17-NH2) or the CTFP (C-Terminus Flanking Peptide) and no interference with various endogenous or exogenous compounds.
The test is linear between 0 and 50 pM hPG80 (native or recombinant). We demonstrated a trueness of measurement, an accuracy and a variability of hPG80 quantification with the DxPG80 test below the 20% relative errors as recommended in the guidelines. The limit of detection of hPG80and the limit of quantification were calculated as 1 pM and 3.3 pM respectively. In conclusion, these results show the strong analytical performance of the DxPG80 test to measure hPG80 in blood samples.
Cappellini Monica, Flaceliere Maud, Saywell Veronique, Soule Julien, Blanc Emilie, Belouin Fanny, Ortiz Erika, Canterel-Thouennon Lucile, Poupeau Sophie, Tigrett Sylvia, Vire Bérengère, Liaud Pierre, Blairvacq Mélina, Joubert Dominique, Prieur Alexandre